GAS-EXCHANGE DYNAMICS OF GLYCEROLIZED FROZEN BLOOD

Fetal blood sampling and gas exchange.

Frozen Rydberg Gas:

A frozen Rydberg gas is a sample of atoms with highly excited electronic states and very low temperature. In our experiments, Rb atoms are cooled and trapped in a magneto-optical trap (MOT) (fig.1&2), and excited to the Rydberg states (the principle quantum number n ~30) by pulsed dye lasers (fig.3). In such a system, the density of Rydberg atoms Nd is typically ~10atoms/cm, and the temperature...

Blood biocompatibility assessment of an intravenous gas exchange device.

To treat acute lung failure, an intravenous membrane gas exchange device, the Hattler Catheter, is currently under development. Several methods were employed to evaluate the biocompatibility of the device during preclinical testing in bovines, and potential coatings for the fibers comprising the device were screened for their effectiveness in reducing thrombus deposition in vitro. Flow cytometr...

The rate of intrapulmonary blood gas exchange in living animals.

Use of frozen blood.

Frozen blood is most easily and economically prepared using high glycerol (40 percent). Storage is at —80° in a mechanical freezer. Thawing must be rapid and washing can be carried out with low electrolyte sugar solutions and agglomeration or with high electrolyte solutions and continuous flow centrif­ ugation. These processed cells have many advantages not found in raw routinely stored blood. ...